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fech  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology fech
    Fech, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fech/pm41872136-238-23-27?v=Santa+Cruz+Biotechnology
    Average 94 stars, based on 50 article reviews
    fech - by Bioz Stars, 2026-07
    94/100 stars

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    Validation of proteomics-identified protein target with Western blot. ( A ) Western blot analysis of RIPA-extracted WT, HET, and SMA liver samples (n = 3 per group), using antibodies against ferrochelatase (FECH), survival motor neuron (SMN) protein and Vinculin (which served as loading control). ( B ) Quantification of FECH normalized to Vinculin, expressed as fold change relative to WT. Bars represent ± SD. Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test (ns = not significant, ****p < 0.0001).

    Journal: Data in Brief

    Article Title: Proteomics dataset of liver tissue from spinal muscular atrophy, heterozygous, and wild-type mice, enabling pathway identification

    doi: 10.1016/j.dib.2026.112632

    Figure Lengend Snippet: Validation of proteomics-identified protein target with Western blot. ( A ) Western blot analysis of RIPA-extracted WT, HET, and SMA liver samples (n = 3 per group), using antibodies against ferrochelatase (FECH), survival motor neuron (SMN) protein and Vinculin (which served as loading control). ( B ) Quantification of FECH normalized to Vinculin, expressed as fold change relative to WT. Bars represent ± SD. Statistical analysis was performed using one-way ANOVA with Tukey’s post hoc test (ns = not significant, ****p < 0.0001).

    Article Snippet: The blot was incubated for SMN (same as above), FECH (rabbit, proteintech, Cat#: 14466-1-AP, dilution 1:1000), and vinculin (rabbit, Cat#:129002, Abcam, dilution 1:1000) served as the loading control.

    Techniques: Biomarker Discovery, Western Blot, Control